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PD Dr. M. Eckhardt  -  Publikationsliste

2022

  • Jordans, S., Hardt, R., Becker, I., Winter, D., Wang-Eckhardt, L., and Eckhardt, M. (2022): Age-dependent increase in Schmidt-Lanterman incisures and a Cadm4 associated membrane skeletal complex in fatty acid 2-hydroxylase deficient mice, a mouse model of spastic paraplegia SPG35. Mol. Neurobiol. 59:3969-3979
  • Wang-Eckhardt, L., Sylvester, M., Becker, I., Allam, J.-P., and Eckhardt, M. (2022): Citrylglutamate synthase deficient male mice are subfertile with impaired histone and transition protein 2 removal in late spermatids. Biochem. J. 479:953-972
  • Wang-Eckhardt, L., Becker, I., Wang, Y., Yuan, J., and Eckhardt, M. (2022): Absence of endogenous carnosine synthesis does not increase protein carbonylation and advanced lipoxidation end products in brain, kidney or muscle. Amino Acids 54:1013-1023

2021

  • Wang-Eckhardt, L., Becker, I., and Eckhardt, M. (2021): The PGRMC1 antagonist AG-205 inhibits synthesis of galactosylceramide and sulfatide. Cells 10:3520
  • Qi, L., Martin-Sandoval, M.S., Mathews, T.P., Gu, W., Eckhardt, M., Zhao, Z., Agathocleous, M., and Morrison, S.J. (2021): Aspartate is limiting for hematopoietic stem cell function. Cell Stem Cell 28:1982-1999
  • Becker, I., Wang-Eckhardt, L., Lodder-Gadaczek, J., Wang, Y. Grünewald, A., and Eckhardt, M. (2021) Mice deficient in the NAAG synthetase II gene Rimkla are impaired in a novel object recognition task. J. Neurochem. 157:2008-2023

2020

  • Hardt, R., Jordans, S., Winter, D., Gieselmann, V., Wang-Eckhardt, L., and Eckhardt, M. (2020): Decreased turnover of the CNS myelin protein Opalin in a mouse model of hereditary spastic paraplegia 35. Hum. Mol. Genet. 29:3616-3630
  • Wang-Eckhardt, L., Bastian, A., Bruegmann, T., Sasse, P., and Eckhardt, M. (2020): Carnosine synthase deficiency is compatible with normal skeletal muscle and olfactory function but causes reduced olfactory sensitivity in aging mice. J. Biol. Chem. 295:17100-17113
  • Wang-Eckhardt, L., and Eckhardt M. (2020): A progesterone receptor membrane component 1 antagonist induces large vesicles independent of progesterone receptor membrane component 1 expression. Biol. Chem. 401:1093-1099
  • Li, L., Pu, Q., Hintze, M., Wang, Y., Eckhardt, M., Gieselmann, V., Tiemann, I., Qi, X., Cai, D., Wang, J., and Huang, R. (2020): BDNF and NGF signals originating from sensory ganglia promote cranial motor axon growth. Exp. Brain Res. 238:111-119
  • Nagarajan, B., Harder, A., Japp, A., Häberlein, F., Mingardo, E., Kleinert, H., Yilmaz, Ö., Zoons, A., Rau, B., Christ, A., Kubitscheck, U., Eiberger, B., Sandhoff, R., Eckhardt, M., Hartmann, D., and Odermatt, B. (2020): CNS myelin protein 36K regulates oligodendrocyte differentiation through Notch. Glia 68:509-527

2019

  • Hofer, D.C., Zirkovits, G., Pelzmann, H.J., Huber, K., Pessentheiner, A.R., Xia, W., Uno, K., Miyazaki, T., Kon, K., Tsuneki, H., Pendl, T., Al Zoughbi, W., Madreiter-Sokolowski, C.T., Trausinger, G., Abdellatif, M., Schoiswohl, G., Schreiber, R., Eisenberg, T., Magnes, C., Sedej, S., Eckhardt, M., Sasahara, M., Sasaoka, T., Nitta, A., Hoefler, G., Graier, W.F., Kratky, D., Auwerx, J., and Bogner-Strauss, J.G. (2019): N-acetylaspartate availability is essential for juvenile survival on fat-free diet and determines metabolic health. FASEB J. 33:13808-13824
  • Fewou, S.N., Röckle, I., Hildebrandt, H., and Eckhardt, M. (2019): Transgenic overexpression of polysialyltransferase ST8SiaIV under the control of a neuron-specific promoter does not affect brain development but impairs exploratory behavior. Glycobiology 29:657-668

2010 - 2018

  • Hardt, R., Winter, D., Gieselmann, V., and Eckhardt, M. (2018): Identification of progesterone receptor membrane component-1 as an interaction partner and possible regulator of fatty acid 2-hydroxylase. Biochem. J. 475:853-871
  • von Gerichten, J., Schlosser, K., Lamprecht, D., Morace, I., Eckhardt, M., Wachten, D., Jennemann, R., Gröne, H.-J., Mack, M., and Sandhoff, R. (2017): Diastereomer-specific quantification of bioactive hexosylceramides from bacteria and mammals. J. Lipid Res. 58:1247-1258
  • Eckhardt, M., and Wang-Eckhardt, L. (2015): A commercial human protamine-2 antibody used in several studies to detect mouse protamine-2 recognizes mouse transition protein-2 but not protamine-2. Mol. Hum. Reprod. 21:825-831
  • Maier, H., Wang-Eckhardt, L., Hartmann, D., Gieselmann, V., and Eckhardt, M. (2015): N-acetylaspartate synthase deficiency corrects the myelin phenotype in a Canavan disease mouse model but does not affect survival time. J. Neurosci. 35:14501-14516
  • Doerr, J., Böckenhoff, A., Ewald, B., Ladewig, J., Eckhardt, M., Gieselmann, V., Matzner, U., Brüstle, O., and Koch, P. (2015): Arylsulfatase A overexpressing human iPSC-derived neural cells reduce CNS sulfatide storage in a mouse model of metachromatic leukodystrophy. Mol. Ther. 23:1519-1531
  • Ebel, P., Imgrund, S., Vom Dorp, K., Hofmann, K., Maier, H., Drake, H., Degen, J., Dörmann, P., Eckhardt, M., Franz, T., and Willecke, K. (2014): Ceramide Synthase 4 deficiency in mice causes lipid alterations in sebum and results in alopecia. Biochem. J. 461:147-158
  • Ebel, P., vom Dorp, K., Petrasch-Parwez, E., Zlomuzica, A., Kinugawa, K., Mariani, J., Minich, D., Ginkel, C., Degen, J., Eckhardt, M., Dere, E., Dörmann, P., and Willecke, K. (2013): Inactivation of ceramide synthase 6 in mice results in an altered sphingolipid metabolism and behavioral abnormalities. J. Biol. Chem. 288:21433-21447
  • Lodder-Gadaczek, J., Gieselmann, V., and Eckhardt, M. (2013): Vesicular uptake of N-acetylaspartylglutamate is catalyzed by sialin (SLC17A5). Biochem. J. 454:31-38
  • Bakhti, M., Snaidero, N., Schneider, D., Aggarwal, S., Möbius, W., Janshoff, A., Eckhardt, M., Nave, K.A., and Simons, M. (2013): Loss of electrostatic cell-surface repulsion mediates myelin membrane adhesion and compaction in the central nervous system. Proc. Natl. Acad. Sci. USA 110:3143-3148
  • Ginkel, C., Hartmann, D., vom Dorp, K., Zlomuzica, A., Farwanah, H., Eckhardt, M., Sandhoff, R., Degen, J., Rabionet, M., Dere, E., Dörmann, P., Sandhoff, K., and Willecke, K. (2012): Ablation of neuronal ceramide synthase 1 in mice decreases ganglioside levels and expression of myelin associated glycoprotein in oligodendrocytes. J. Biol. Chem. 287:41888-41902
  • Matthes, F., Stroobants, S., Gerlach, D., Wohlenberg, C., Wessig, C., Fogh, J., Gieselmann, V., Eckhardt, M., D'Hooge, R., and Matzner, U. (2012): Efficacy of enzyme replacement therapy in an aggravated mouse model of metachromatic leukodystrophy declines with age. Hum. Mol. Genet. 21:2599-2609
  • Kuronen, M., Hermansson, M., Manninen, O., Zech, I., Talvitie, M., Laitinen, T., Gröhn, O., Somerharju, P., Eckhardt, M., Cooper, J., Lehesjoki, A.-E., Lahtinen, U., and Kopra, O. (2012): Galactolipid deficiency characterizes the early pathogenesis of the neuronal ceroid lipofuscinosis model Cln8mnd: implications to delayed myelination and oligodendrocyte maturation. Neuropathol. Appl. Neurobiol. 38:471-486
  • Jungnickel, J., Eckhardt, M., Haastert, K., Claus, P., Bronzlik, P., Lipokatic-Takacs, E., Maier, H., Gieselmann, V., and Grothe, C. (2012): Schwann cell-derived polysialic acid mediates different effects during peripheral nerve regeneration. Glycobiology 22:107-115
  • Maier, H., Meixner, M., Hartmann, D., Sandhoff, R., Wang-Eckhardt, L., Zöller, I., Gieselmann, V., and Eckhardt, M. (2011): Normal fur development and sebum production depends on fatty acid 2-hydroxylase expression in sebaceous glands. J. Biol. Chem. 286:25922-25934
  • Marsching, C., Eckhardt, M., Gröne, H.-J., Sandhoff, R., and Hopf, C. (2011): Imaging of complex sulfatides SM3 and SB1a in mouse kidney using MALDI-TOF/TOF mass spectrometry. Anal. Bioanal. Chem. 401:53-64
  • Faldini, E., Stroobants, S., Lüllmann-Rauch, R., Eckhardt, M., Gieselmann, V., Balschun, D., and D’Hooge, R. (2011): Telencephalic histopathology and changes in behavioural and neural plasticity in a murine model for metachromatic leukodystrophy. Behav. Brain Res. 222:309-314
  • Lodder-Gadaczek, J., Becker, I., Gieselmann, V., Wang-Eckhardt, L., and Eckhardt, M. (2011): N-acetylaspartylglutamate synthetase-II synthesizes N-acetylaspartyl-glutamylglutamate. J. Biol. Chem. 286:16693-16706
  • Meixner, M., Jungnickel, J., Grothe, C., Gieselmann, V., and Eckhardt, M. (2011): Myelination in the absence of UDP-galactose:ceramide galactosyltransferase and fatty acid 2-hydroxylase. BMC Neurosci. 12:22
  • Henseler, D., Turner, J., Eckhardt, M., van der Mark, M., Revsin, Y., Lin, M., Kranz, T., and Meyer, J. (2011): Unusual 5'-regulatory structure and regulation of the murine Mlc1 gene: lack of promoter-specific functional elements. J. Nucleic Acids Investig. 2:70-76 (e11)
  • Eckhardt, M. (2010): Pathology and current treatment of neurodegenerative sphingolipidoses. Neuromolecular Med. 12:362-382
  • Becker, I., Lodder, J., Gieselmann, V., and Eckhardt, M. (2010): Molecular characterization of N-acetylaspartylglutamate synthetase. J. Biol. Chem. 285:29156-29164 
  • Dick, K.J., Eckhardt, M., Paisán-Ruiz, C., Alkhayat Alshehhi, A., Proukakis, C., Sibtain, N., Maier, H., Sharifi, R., Patton, M.A., Bashir, W., Koul, R., Raeburn, S.A., Gieselmann, V., Houlden, H., and Crosby, A.H. (2010): Mutation of fatty acid 2-hydroxylase underlies a complicated form of hereditary spastic paraplegia (SPG35). Hum. Mutat. 31:E1251-E1260
  • van Zyl, R., Gieselmann, V., and Eckhardt, M. (2010): Elevated sulfatide levels in neurons causes lethal audiogenic seizures in mice. J. Neurochem. 112:282-295
  • 2001 - 2009
  • Imgrund, S., Hartmann, D., Farwanah, H., Eckhardt, M., Sandhoff, R., Degen, J., Gieselmann, V., Sandhoff, K., and Willecke K. (2009): Adult ceramide synthase 2 (CerS2) deficient mice exhibit myelin sheath defects, cerebellar degeneration, and hepatocarcinomas. J. Biol. Chem. 284:33549-33560
  • Zöller, I., Meixner, M., Hartmann, D., Büssow, H., Meyer, R., Gieselmann, V., and Eckhardt, M. (2008): Absence of 2-hydroxylated sphingolipids is compatible with normal neural development but causes late-onset axon and myelin sheath degeneration. J. Neurosci. 28:9741-9754
  • Stroobants, S., Leroy, T., Eckhardt, M., Aerts, J.-M., Berckmans, D., and D'Hooge, R. (2008): Early signs of neurolipidosis-related behavioural alterations in a murine model of metachromatic leukodystrophy. Behav. Brain Res. 189:306-316
  • Eckhardt, M. (2008): The role and metabolism of sulfatide in the nervous system. Mol. Neurobiol. 37:93-103
  • Becker, I., Wang-Eckhardt, L., Yaghootfam, A., Gieselmann, V., and Eckhardt, M. (2008): Differential expression of (dihydro)ceramide synthases in mouse brain: oligodendrocyte-specific expression of CerS2/Lass2. Histochem. Cell Biol. 129:233-241
  • Townson, K., Greenshields, K., Veitch, J., Nicholl, D., Eckhardt, M., Galanina, O., Bovin, N., Samain, E., Antoine, T., Bundle, D., Zhang, P., Chun Ling, C., and Willison, H.J. (2007): Sulfatide binding properties of murine and human anti-ganglioside antibodies. Glycobiology 17:1156-1166
  • Ramakrishnan, H., Khalaj-Hedayati, K., Lüllmann-Rauch, R., Wessig, C., Fewou, S.N., Maier, H., Goebel, H.H., Gieselmann, V., and Eckhardt, M. (2007): Increasing sulfatide synthesis in myelin-forming cells of arylsulfatase A-deficient mice causes demyelination and neurological symptoms reminiscent of human metachromatic leukodystrophy. J. Neurosci. 27:9482-9490
  • Eckhardt, M., Khalaj-Hedayati, K., Pitsch, J., Lüllmann-Rauch, R., Beck, H., Fewou, S.N., and Gieselmann, V. (2007): Sulfatide storage in neurons causes hyperexcitability and axonal degeneration in a mouse model of metachromatic leukodystrophy. J. Neurosci. 27:9009-9021
  • Yaghootfam, A., Sorkalla, T., Häberlein, H., Gieselmann, V., Kappler, J., and Eckhardt, M. (2007): Cerebroside sulfotransferase forms homodimers in living cells. Biochemistry 46:9260-9269
  • Fewou, S.N., Ramakrishnan, H., Büssow, H., Gieselmann, V., and Eckhardt, M. (2007): Down-regulation of polysialic acid is required for efficient myelin formation. J. Biol. Chem. 282:16700-16711
  • Lütjohann, D., Harzer, K., Gieselmann, V., and Eckhardt, M. (2006): Reduced brain cholesterol content in arylsulfatase A-deficient mice. Biochem. Biophys. Res. Commun. 344:647-650
  • Tiralongo, J., Ashikov, A., Routier, F., Eckhardt, M., Bakker, H., Gerardy-Schahn, R., and von Itzstein, M. (2006): Functional expression of the CMP-sialic acid transporter in Escherichia coli and its identification as a simple mobile carrier. Glycobiology 16:73-81
  • Zöller, I., Büssow, H., Gieselmann, V., and Eckhardt, M. (2005): Oligodendrocyte specific ceramide galactosyltransferase (CGT) expression phenotypically rescues CGT deficient mice and demonstrates that CGT activity does not limit brain galactosylceramide level. Glia 52:190-198
  • Fewou, S.N., Büssow, H., Schaeren-Wiemers, N., Vanier, M.T., Macklin, W.B., Gieselmann, V., and Eckhardt, M. (2005): Reversal of non-hydroxy:α-hydroxy galactosylceramide ratio and unstable myelin in transgenic mice overexpressing UDP-galactose:ceramide galactosyltransferase. J. Neurochem. 94:469-481
  • Eckhardt, M., Yaghootfam, A., Fewou, S.N., Zöller, I., and Gieselmann, V. (2005): A mammalian fatty acid hydroxylase responsible for the formation of α-hydroxylated galactosylceramide in myelin. Biochem. J. 388:245-254
  • Yaghootfam, A., Gieselmann, V., and Eckhardt, M. (2005): Delay of myelin formation in arylsulfatase A deficient mice. Eur. J. Neurosci. 21:711-720
  • Eckhardt, M., Fewou, S.N., Ackermann, I., and Gieselmann, V. (2002): N-glycosylation is required for full enzymic activity of the murine galactosylceramide sulphotransferase. Biochem. J. 368:317-324
  • Lühn, K., Wild, M.K., Eckhardt, M., Gerardy-Schahn, R., and Vestweber, D. (2001): The defective gene in leukocyte adhesion deficiency II encodes a putative GDP-fucose transporter. Nat. Genet. 28:69-72
  • 1994 - 2000
  • Windfuhr, M., Manegold, A., Mühlenhoff, M., Eckhardt, M., and Gerardy-Schahn, R. (2000): Molecular defects that cause loss of polysialic acid in the complementation group 2A10. J. Biol. Chem. 275:32861-32870
  • Eckhardt, M., Bukalo, O., Chazal, G., Wang, L., Goridis, C., Schachner, M., Gerardy-Schahn, R., Cremer, H., and Dityatev, A. (2000): Mice deficient in the polysialyltransferase ST8SiaIV/PST-1 allow discrimination of the roles of neural cell adhesion molecule protein and polysialic acid in neural development and synaptic plasticity. J. Neurosci. 20:5234-5244
  • Eckhardt, M., Barth, H., Blöcker, D., and Aktories, K. (2000): Binding of Clostridium botulinum C2 toxin to asparagine-linked complex and hybrid carbohydrates. J. Biol. Chem. 275:2328-2334
  • Gerardy-Schahn, R., and Eckhardt, M. (2000): Nucleotide Sugar Transporters. In: Beat Ernst, Gerald W. Hart, Pierre Sinaý (Editors): Carbohydrates in Chemistry and Biology: A Comprehensive Handbook. (Part II, 3.), Chapter 41, pp. 19-36; Wiley-VCH Verlag GmbH, Weinheim, Germany (doi: 10.1002/9783527618255.ch41); Print ISBN: 9783527295111; Online ISBN: 9783527618255
  • Eckhardt, M., Gotza, B., and Gerardy-Schahn, R. (1999): Membrane topology of the mammalian CMP-sialic acid transporter. J. Biol. Chem. 274:8779-8787
  • Eckhardt, M., and Gerardy-Schahn, R. (1998): Genomic organization of the murine polysialyltransferase gene ST8SiaIV (PST-1). Glycobiology 8:1165-1172
  • Münster, A.K., Eckhardt, M., Potvin, B., Mühlenhoff, M., Stanley, P., and Gerardy-Schahn, R. (1998): Mammalian cytidine 5´-monophosphate N-acetylneuraminic acid synthetase: A nuclear protein with evolutionarily conserved structural motifs. Proc. Natl. Acad. Sci. USA 95:9140-9145
  • Eckhardt, M., Gotza, B., and Gerardy-Schahn, R. (1998): Mutants of the CMP-sialic acid transporter causing the Lec2 phenotype. J. Biol. Chem. 273:20189-20195
  • Mühlenhoff, M., Eckhardt, M., and Gerardy-Schahn, R. (1998): Polysialic acid: three-dimensional structure, biosynthesis, and function. Curr. Opin. Struct. Biol. 8:558-564
  • Glüer, S, Schelp, C., Madry, N., von Schweinitz, D., Eckhardt, M., and Gerardy-Schahn, R. (1998): Serum polysialylated neural cell adhesion molecule in childhood neuroblastoma. Br. J. Cancer 78:106-110
  • Eckhardt, M., and Gerardy-Schahn, R. (1997): Molecular cloning of the hamster CMP-sialic acid transporter. Eur. J. Biochem. 248:187-192
  • Berninsone, P., Eckhardt, M., Gerardy-Schahn, R., and Hirschberg, C.B. (1997): Funtional expression of the murine Golgi CMP-sialic acid transporter in Saccharomyces cerevisiae. J. Biol. Chem. 272:12616-12619
  • Mühlenhoff, M., Eckhardt, M., Bethe, A., Frosch, M., and Gerardy-Schahn, R. (1996): Autocatalytic polysialylation of polysialyltransferase-1. EMBO J. 15:6943-6950
  • Mühlenhoff, M., Eckhardt, M., Bethe, A., Frosch, M., and Gerardy-Schahn, R. (1996): Polysialylation of NCAM by a single enzyme. Curr. Biol. 6:1188-1191
  • Eckhardt, M., Mühlenhoff, M., Bethe, A., and Gerardy-Schahn, R. (1996): Expression cloning of the Golgi CMP-sialic acid transporter. Proc. Natl. Acad. Sci. USA 93:7572-7576
  • Gerardy-Schahn, R., Bethe, A., Brennecke, T., Mühlenhoff, M., Eckhardt, M., Ziesing, S., Lottspeich, F., and Frosch, M. (1995): Molecular cloning and functional expression of bacteriophage PK1E encoded endoneuraminidase EndoNE. Mol. Microbiol. 16:441-450
  • Eckhardt, M., Mühlenhoff, M., Bethe, A., Koopman, J., Frosch, M., and Gerardy-Schahn, R. (1995): Molecular characterization of eukaryotic polysialyltransferase-1. Nature 373:715-718
  • Gerardy-Schahn, R., Eckhardt, M., Ledermann, J., and Kemshead, J.T. (1994): Topography of NCAM antigenic epitopes recognized by SCLC cluster-1 antibodies. A consensus view. Int. J. Cancer, Suppl. 8:27-29
  • Gerardy-Schahn, R., and Eckhardt, M. (1994): Hot spots of antigenicity in the neural cell adhesion molecule NCAM. Int. J. Cancer, Suppl. 8:38-42
  • Madry, N. (1994): Characterization of tumor-associated neural cell adhesion molecule in human serum. Cancer Res. 54:2598-2603